Fifth complement cascade protein (C5) cleavage fragments disrupt the [HTML] f SDF-1/CXCR4 axis: further evidence that innate immunity orchestrates

Fifth complement cascade protein (C5) cleavage fragments disrupt the [HTML] f SDF-1/CXCR4 axis: further evidence that innate immunity orchestrates
the mobilization of hematopoietic stem …
Authors Ali Jalili, Neeta Shirvaikar, Leah Marquez-Curtis, Yuanyuan Qiu, Chris Korol, HakMo Lee, A Robert Turner, Mariusz Z Ratajczak, Anna Janowska-Wieczorek
Publication date 2010/4/1
Journal Experimental hematology
Volume 38
Issue 4
Pages 321-332
Publisher Elsevier
Description OBJECTIVE:
Having previously demonstrated that the complement system modulates mobilization of hematopoietic stem/progenitor cells (HSPC) in mice, we investigated the involvement of C5 cleavage fragments (C5a/desArg C5a) in human HSPC mobilization.
MATERIALS AND METHODS:
C5 cleavage fragments in the plasma were evaluated by enzyme-linked immunosorbent assay using human anti-desArgC5a antibody, and expression of the C5a/desArgC5a receptor (CD88) in hematopoietic cells by flow cytometry. We also examined the chemotactic responses of hematopoietic cells to C5 cleavage fragments and expression of stromal cell-derived factor-1 (SDF-1)-degrading proteases that perturb retention of HSPC in bone marrow, namely matrix metalloproteinase (MMP)-9, membrane type (MT) 1-MMP, and carboxypeptidase M.
RESULTS:
We found that plasma levels of desArg C5a are significantly higher …
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