The HGFc-Met axis synergizes with G-CSF in the mobilization of hematopoietic stemprogenitor cells

The HGF/c-Met axis synergizes with G-CSF in the mobilization of hematopoietic stem/progenitor cells
Authors Ali Jalili, Neeta Shirvaikar, Leah A Marquez-Curtis, A Robert Turner, Anna Janowska- Wieczorek
Publication date 2010/8/1
Journal Stem cells and development
Volume 19
Issue 8
Pages 1143-1151
Publisher Mary Ann Liebert, Inc.
Description As granulocyte-colony-stimulating factor (G-CSF)-induced mobilization of hematopoietic stem/progenitor cells (HSPCs) increases human serum levels of hepatocyte growth factor (HGF), our aim was to investigate the role of HGF and its receptor, c-Met, in the mobilization of HSPC. CD34+ cells and leukocytes were isolated from the bone marrow (BM) of normal donors and the peripheral blood (PB) of patients mobilized with G-CSF and chemotherapy. Plasma HGF levels were evaluated by ELISA and HGF and c-Met expression by RT-PCR, fluorescence-activated cell sorter (FACS) analysis, and confocal microscopy. Because matrix metalloproteinases (MMPs) facilitate migration across extracellular matrix (ECM) and basement membranes, we also examined expression of MMP-9 and membrane type 1 (MT1)-MMP in hematopoietic cells after HGF stimulation. We found that plasma HGF levels in mobilized (m)PB…
Total citations Cited by 41
Scholar articles The HGF/c-Met axis synergizes with G-CSF in the mobilization of hematopoietic stem/progenitor cells
A Jalili, N Shirvaikar, LA Marquez-Curtis, AR Turner… – Stem cells and development, 2010
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