Induction of HM1. 24 peptide-specific cytotoxic T lymphocytes by using [HTI peripheral-blood stem-cell harvests in patients with multiple myeloma

Induction of HM1. 24 peptide-specific cytotoxic T lymphocytes by using [HTI peripheral-blood stem-cell harvests in patients with multiple myeloma
Authors Ali Jalili, Shuji Ozaki, Tomoko Hara, Hironobu Shibata, Toshihiro Hashimoto, Masahiro Abe, Yasuhiko Nishioka, Toshio Matsumoto
Publication date 2005/11/15
Journal Blood
Volume 106
Issue 10
Pages 3538-3545
Publisher American Society of Hematology
Description HM1.24 antigen is preferentially overexpressed in multiple myeloma (MM) cells but not in normal cells. To explore the potential of HM1.24 as a target for cellular immunotherapy, we selected 4 HM1.24-derived peptides that possess binding motifs for HLA-A2 or HLA- A24 by using 2 computer-based algorithms. The ability of these peptides to generate cytotoxic T lymphocytes (CTLs) was examined in 20 healthy donors and 6 patients with MM by a reverse immunologic approach. Dendritic cells (DCs) were induced from peripheral-blood mononuclear cells of healthy donors or peripheral-blood stem-cell (PBSC) harvests from patients with MM, and autologous CD8+ T cells were stimulated with HM1.24 peptide-pulsed DCs. Both interferon-y-producing and cytotoxic responses were observed after stimulation with either HM1.24-126 or HM1.24-165 peptides in HLA- A2 or HLA-A24 individuals. The peptide-specific…
Total citations Cited by 53
Scholar articles Induction of HM1. 24 peptide-specific cytotoxic T lymphocytes by using peripheral-blood stem-cell harvests in patients with multiple myeloma
A Jalili, S Ozaki, T Hara, H Shibata, T Hashimoto… – Blood, 2005
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