The Disruption of Mage-11 Gene via CRISPR/Cas9 Method Induced Apoptosis in the in vitro Model of Prostate Cancer

The Disruption of Mage-11 Gene via CRISPR/Cas9 Method Induced Apoptosis in the in vitro Model of Prostate Cancer.
Authors Leila Farhadi, Farzad Soleimani, Shohreh Fakhari, Ali Jalili
Publication date 2023/1/1
Journal The Gulf journal of oncology
Volume 1
Issue 41
Pages 7-16
Description Materials and methods
First, MAGE-A11 gene was knocked out in PC-3 cell line using” Clustered regularly interspaced short palindromic repeats”(CRISPR)/” CRISPR-associated genes 9″ (CRISPR/Cas9) method. Next, the expression levels of MAGE-A11, survivin and Ribonucleotide Reductase Small Subunit M2 (RRM2) genes were determined by quantitative polymerase chain reaction (qPCR) technique. The levels of proliferation and apoptosis were also analyzed in PC-3 cells using CCK-8 and Annexin V-PE/7-AAD assays.
Results
The results showed that the disruption of MAGE-A11 by CRISPR/Cas9 method significantly decreased proliferation (P<0.0001) and enhanced apoptosis (P< 0.05) in PC-3 cells compared to control group. Moreover, the disruption of MAGE-A11 significantly down regulated the expression levels of survivin and RRM2 genes (P< 0.05).
Conclusion
Our results demonstrated that knocking out MAGE-11 gene by CRISPR/CAS9 technique could efficiently inhibit cell proliferation and induce apoptosis in PC3 cells. Survivin and RRM2 genes might also participated in these processes.
Scholar articles The Disruption of Mage-11 Gene via CRISPR/Cas9 Method Induced Apoptosis in the in vitro Model of Prostate Cancer.
L Farhadi, F Soleimani, S Fakhari, A Jalili – The Gulf journal of oncology, 2023 All 4 versions