Carboxypeptidase M Expressed by Human Bone Marrow Cells Cleaves the C- Terminal Lysine of Stromal Cell-Derived Factor-1a Another Player in Hematopoietic

Carboxypeptidase M Expressed by Human Bone Marrow Cells Cleaves the C- Terminal Lysine of Stromal Cell-Derived Factor-1a: Another Player in
Hematopoietic …
Authors Leah Marquez-Curtis, Ali Jalili, Kathleen Deiteren, Neeta Shirvaikar, Anne-Marie
Lambeir, Anna Janowska-Wieczorek
Publication date 2008/5
Journal Stem cells
Volume 26
Issue 5
Pages 1211-1220
Publisher Oxford University Press
Description Carboxypeptidase M (CPM) is a membrane-bound zinc-dependent protease that cleaves C-terminal basic residues, such as arginine or lysine, from peptides/proteins. We examined whether CPM is expressed by hematopoietic and stromal cells and could degrade stromal cell-derived factor (SDF)-1a, a potent chemoattractant for hematopoietic stem/progenitor cells (HSPC). We found that (a) CPM transcript is expressed by bone marrow (BM) and mobilized peripheral blood CD34+ cells, myeloid, erythroid, and megakaryocytic cell progenitors, mononuclear cells (MNC), polymorphonuclear cells (PMN), and stromal cells, including mesenchymal stem cells; and that (b) granulocyte- colony-stimulating factor (G-CSF) significantly increases its expression at the gene and protein levels in MNC and PMN. Moreover, we found that recombinant CPM cleaves full- length SDF-1a (1-68) rapidly, removing the C-terminal…
Total citations Cited by 71
Scholar articles Carboxypeptidase M expressed by human bone marrow cells cleaves the C-terminal lysine of stromal cell-derived factor-1 a: another player in hematopoietic stem/progenitor cell mobilization?
L Marquez-Curtis, A Jalili, K Deiteren, N Shirvaikar… – Stem cells, 2008
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